Quelpr

Aspects of Genetic Engineering · CAPE Biology Unit 1

47 past-paper questions on Aspects of Genetic Engineering, part of Genetics, Variation and Natural Selection, from every CAPE Biology Unit 1 paper on Quelpr.

Practise Aspects of Genetic Engineering
  1. 5(a)5 marks· Biology · Unit 1 Q5 5(a)Explain what is meant by the term 'gene'. Outline the principles of using restriction enzymes to cut a gene from a length of DNA. Note: Details of the steps involved in recombinant DNA technology are not required.
  2. 5(a)7 marks· Biology · Unit 1 Q5 5(a)Evaluate the use of genetically modified organisms in agriculture. Include an explanation of the term 'genetically modified organism'.
  3. 5(a)(i)2 marks· Biology · Unit 1 Q5 5(a)(i)Explain the terms 'vector' and 'recipient' as applied to genetic engineering.
  4. 5(a)(ii)4 marks· Biology · Unit 1 Q5 5(a)(ii)Discuss the role played by E. coli (as a vector and recipient) in the production of insulin, utilizing genetic techniques.
  5. 5(b)4 marks· Biology · Unit 1 Q5 5(b)Outline the FOUR key steps involved in producing a recombinant piece of gene.
  6. 5(b)8 marks· Biology · Unit 1 Q5 5(b)Explain the steps involved in the use of recombinant DNA technology for the production of human insulin from bacteria under the following headings: Gene isolation; Gene insertion into a vector.
  7. 5(b)1 mark· Biology · Unit 1 Q5 5(b)Gene therapy is the application of the principle of genetic engineering in the treatment of diseases.
  8. 5(b)(i)5 marks· Biology · Unit 1 Q5 5(b)(i)Outline the principle of genetic engineering, including a brief description of the basic steps of this technique.
  9. 5(b)(i)2 marks· Biology · Unit 1 Q5 5(b)(i)Give a brief description of one human disease for which the use of somatic gene therapy has been shown to be effective.
  10. 5(b)(i)4 marks· Biology · Unit 1 Q5 5(b)(i)Outline the procedure of somatic gene therapy, giving FOUR key steps.
  11. 5(b)(i)2 marks· Biology · Unit 1 Q5 5(b)(i)Explain the role of 'restriction enzymes' in bacterial cells.
  12. 5(b)(ii)5 marks· Biology · Unit 1 Q5 5(b)(ii)Despite its promising potential, one of the arguments sometimes raised against the use of gene therapy is that it is technically too dangerous. With reference to cystic fibrosis, discuss reasons for this viewpoint.
  13. 5(b)(ii)2 marks· Biology · Unit 1 Q5 5(b)(ii)With reference to cystic fibrosis, comment on ONE difficulty in using gene therapy to treat diseases.
  14. 5(b)(ii)8 marks· Biology · Unit 1 Q5 5(b)(ii)Discuss FOUR reasons why somatic gene therapy, despite its potential, is still not used as an effective treatment for human diseases.
  15. 5(b)(ii)4 marks· Biology · Unit 1 Q5 5(b)(ii)Comment on the role of restriction enzymes in genetic engineering.
  16. 5(c)6 marks· Biology · Unit 1 Q5 5(c)Discuss THREE potential threats to humans, and other organisms, of the use of genetically modified crops. Include a definition of the term ‘genetically modified organism'.
  17. 5(c)4 marks· Biology · Unit 1 Q5 5(c)In general terms, discuss FOUR major issues associated with applications of genetic engineering.
  18. Q101 mark · multiple choice· CAPE Biology Unit 1 · 2008 (Rest of Region) · Paper 1Item 10 refers to the figure below which shows the structure of a bacterial cell as seen using an electron microscope. The structure involved in genetic engineering is
  19. Q241 mark · multiple choice· CAPE Biology Unit 1 · 2008 (Rest of Region) · Paper 1Restriction enzymes cut DNA molecules only at specific target sites with particular base sequences. The target sites for some restriction enzymes are shown in the table below. \begin{tabular}{|c|c|} \hline…
  20. Q251 mark · multiple choice· CAPE Biology Unit 1 · 2008 (Rest of Region) · Paper 1In addition to the human insulin gene, which of the following would be required to provide a recombinant plasmid?
  21. Q281 mark · multiple choice· CAPE Biology Unit 1 · 2008 (Rest of Region) · Paper 1In DNA recombinant technology specific genes are harvested then replicated. Which sequence BEST represents the correct sequence of insulin production?
  22. Q291 mark · multiple choice· CAPE Biology Unit 1 · 2008 (Rest of Region) · Paper 1One implication of the use of genetically modified organisms on humans is
  23. Q251 mark · multiple choice· CAPE Biology Unit 1 · 2009 · Paper 1Which of the following may be used as methods of gene transfer? I. Virus II. Plasmid III. Electroporation IV. DNA gun
  24. Q291 mark · multiple choice· CAPE Biology Unit 1 · 2009 · Paper 1A possible benefit of producing transgenic plants is
  25. Q241 mark · multiple choice· CAPE Biology Unit 1 · 2010 · Paper 1Item 24 refers to the following information. Restriction enzymes cut DNA molecules only at specific target sites with particular base sequences. The target sites for some restriction enzymes are shown in the table…
  26. Q251 mark · multiple choice· CAPE Biology Unit 1 · 2010 · Paper 1The function of DNA ligase in genetic engineering is to
  27. Q251 mark · multiple choice· CAPE Biology Unit 1 · 2011 · Paper 1Item 25 refers to the following stages used in the process of genetic engineering. 1. Potential difference applied. 2. Cut by restriction enzymes. 3. DNA extracted from cell. 4. Fragments travel to anode. 5.…
  28. Q261 mark · multiple choice· CAPE Biology Unit 1 · 2011 · Paper 1The function of DNA ligase in genetic engineering is to
  29. Q241 mark · multiple choice· CAPE Biology Unit 1 · 2012 · Paper 1Which restriction enzyme(s) can be used to cut the DNA?
  30. Q251 mark · multiple choice· CAPE Biology Unit 1 · 2012 · Paper 1Which of the following identifies the correct sequence of the process? 1 Potential difference applied. 2 Cut by restriction enzymes. 3 DNA extracted from cell. 4 Fragments travel to anode. 5 ^{32}\text{P} probe added.…
  31. Q291 mark · multiple choice· CAPE Biology Unit 1 · 2012 · Paper 1A possible benefit of producing transgenic plants is
  32. Q251 mark · multiple choice· CAPE Biology Unit 1 · 2013 · Paper 1Which of the following identifies the correct sequence of the process? 1 Potential difference applied. 2 Cut by restriction enzymes. 3 DNA extracted from cell. 4 Fragments travel to anode. 5 ^{32}\text{P} probe…
  33. Q261 mark · multiple choice· CAPE Biology Unit 1 · 2013 · Paper 1A possible benefit of producing transgenic plants is
  34. Q261 mark · multiple choice· CAPE Biology Unit 1 · 2014 · Paper 1A possible benefit of producing transgenic plants is
  35. Q271 mark · multiple choice· CAPE Biology Unit 1 · 2014 · Paper 1Items 27–28 refer to the following map of a plasmid showing the position of recognition sequences of two restriction enzymes. In an experiment to use this plasmid as a vector for the human insulin gene, for which of…
  36. Q281 mark · multiple choice· CAPE Biology Unit 1 · 2014 · Paper 1Items 27–28 refer to the following map of a plasmid showing the position of recognition sequences of two restriction enzymes. In addition to the human insulin gene, which of the following would be required to produce a…
  37. Q261 mark · multiple choice· CAPE Biology Unit 1 · 2015 · Paper 1A possible benefit of producing transgenic plants is
  38. Q271 mark · multiple choice· CAPE Biology Unit 1 · 2015 · Paper 1In the production of recombinant DNA, restriction enzymes are used to cut double stranded DNA molecules to form fragments. Which bonds are cut by these enzymes?
  39. 2(c)(i)3 marks· CAPE Biology Unit 1 · May/June 2021 · Paper 2Analyse the success of the gene therapy treatment for sickle-cell anaemia using the data presented in Figure 3.
  40. 2(c)(ii)12 marks· CAPE Biology Unit 1 · May/June 2021 · Paper 2Discuss three challenges that may limit the success of gene therapy as an effective medical treatment for diseases.
  41. 2(b)(i)2 marks· CAPE Biology Unit 1 · May/June 2024 · Paper 2State TWO features of restriction enzymes.
  42. 2(b)(ii)3 marks· CAPE Biology Unit 1 · May/June 2024 · Paper 2List THREE advantages associated with the use of genetically modified organisms.
  43. 2(b)(iii)3 marks· CAPE Biology Unit 1 · May/June 2024 · Paper 2List THREE disadvantages associated with the use of genetically modified organisms.
  44. 2(b)(iv)5 marks· CAPE Biology Unit 1 · May/June 2024 · Paper 2On the grid provided on page 15, plot the data shown in Table 2 as a line graph and draw a curve of best fit.
  45. 2(c)9 marks· CAPE Biology Unit 1 · May/June 2024 · Paper 2Explain if EACH of the methods shown in Figure 4 can produce DNA that the bacterium E. coli can use to make amylase on a large scale. Present your response in complete paragraphs.
  46. 2(c)(i)2 marks· CAPE Biology Unit 1 · May/June 2025 · Paper 2Define the term 'genetically modified'.
  47. 2(c)(ii)8 marks· CAPE Biology Unit 1 · May/June 2025 · Paper 2Discuss ONE possible social implication and ONE possible environmental implication if farmers were allowed to plant genetically modified soya bean in the Caribbean, using the provided text and data from Table 1 and…

Related topics in Genetics, Variation and Natural Selection