CAPE Biology Unit 1 · 2014 · Paper 1 · Question Q27

Items 27–28 refer to the following map of a plasmid showing the position of recognition sequences of two restriction enzymes. In an experiment to use this plasmid as a vector for the human insulin gene, for which of the following reasons would the genetic engineer choose BamH1 to cut this plasmid?

1. (A)BamH1 makes a staggered cut.
2. (B)To allow identification of the recombinant plasmid.
3. (C)The insulin gene is not complementary to the EcoR1 restriction site.
4. (D)The gene for tetracycline resistance is needed for the expression of the insulin gene.

This question uses a figure or table from the paper — you'll see it when you practise.