CAPE Biology Unit 1 · 2010 · Paper 1 · Question Q24

Item 24 refers to the following information. Restriction enzymes cut DNA molecules only at specific target sites with particular base sequences. The target sites for some restriction enzymes are shown in the table below. [Table] BamHI: G GATCC / CCTAG G EcoRI: G AATTC / C TT AA G HpaI: CC GG / GG CC HpaII: GTT AAC / CAA TTG A small piece of DNA is shown below. GTTAACCCGG CAATTGGGCC Which restriction enzyme(s) can be used to cut the DNA?

1. (A)BamHI, HpaII only
2. (B)EcoRI only
3. (C)HpaI only
4. (D)HpaI and HpaII only

This question uses a figure or table from the paper — you'll see it when you practise.