CAPE Biology Unit 1 · 2008 (Rest of Region) · Paper 1 · Question Q24

Restriction enzymes cut DNA molecules only at specific target sites with particular base sequences. The target sites for some restriction enzymes are shown in the table below. \begin{tabular}{|c|c|} \hline \textbf{Restriction enzyme} & \textbf{Target site} \\ \hline BamHI & \begin{tabular}{l} G GATCC \\ CCTAG G \end{tabular} \\ \hline EcoRI & \begin{tabular}{l} G AATTC \\ CTTAA G \end{tabular} \\ \hline HpaI & \begin{tabular}{l} CC GG \\ GG CC \end{tabular} \\ \hline HpaII & \begin{tabular}{l} GTT AAC \\ CAA TTG \end{tabular} \\ \hline \end{tabular} The diagram below shows a small piece of DNA. GTTAACCCGG CAATTGGGCC Which restriction enzyme(s) can be used to cut the DNA?

1. (A)BamHI, HpaII
2. (B)EcoRI only
3. (C)HpaI only
4. (D)HpaI and HpaII