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2 marksHealth and Disease

CAPE Biology Unit 2 · 2006 · Paper 2 · Question 3(e)(i)

A recombinant plasmid is constructed by inserting a corn protein gene into plasmid A, disrupting its tetracycline resistance gene, while retaining kanamycin resistance. The plasmid is transformed into E. coli.

Briefly outline a method of detection and what you would observe if E. coli has taken up no DNA.

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Other parts of this question

  1. 3(a)Name the type of therapy that the child is undergoing.[1 mark]
  2. 3(b)Identify the type of enzymes used to remove the normal allele from the genome of the donor whose genes are being used in the treatment.[1 mark]
  3. 3(c)Suggest ONE benefit and ONE hazard of the technique shown in Figure 4.[2 marks]
  4. 3(d)Can the change in the genome be passed on to the individual's offspring? Give ONE reason for your answer.[2 marks]
  5. 3(e)(ii)Briefly outline a method of detection and what you would observe if E. coli has taken up recombinant DNA.[2 marks]

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