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6 marksEnzymes

CAPE Biology Unit 1 · 2004 · Paper 2 · Question 5(b)

Trypsin catalyses the hydrolysis of peptide bonds with arginine or lysine residues. Sucrase catalyses hydrolysis of sucrose into glucose and fructose but cannot attack maltose. When sucrase and trypsin are boiled, they become inactive.

Discuss how the above named enzymes exhibit substrate or bond specificity and why they become inactive when boiled.

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Other parts of this question

  1. 5(a)(i)Using graphs and/or suitable examples, describe the effect of the following conditions on enzymes and enzymatic activity: Temperature.[10 marks]
  2. 5(a)(ii)Using graphs and/or suitable examples, describe the effect of the following conditions on enzymes and enzymatic activity: pH.[1 mark]
  3. 5(a)(iii)Using graphs and/or suitable examples, describe the effect of the following conditions on enzymes and enzymatic activity: Enzyme concentration and substrate…[1 mark]
  4. 5(c)Discuss the regulation that is occurring in the metabolic pathway described.[4 marks]

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