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5 marksEnzymes

CAPE Biology Unit 1 · May/June 2025 · Paper 2 · Question 1(a)(iii)

Enzymatic cleavage of biological polymers and energy yield of macromolecules.

Outline a suitable procedure to investigate the activity of amylase on a suspension of starch at 10 °C, 20 °C, 30 °C and 40 °C, including all relevant materials and apparatus and KI/I solution.

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  1. 1(a)(i)Define the term 'activation energy'.[2 marks]
  2. 1(a)(ii)Outline TWO features of triglycerides that facilitate their ability to release more energy per gram than carbohydrates when catabolized.[4 marks]
  3. 1(a)(iv)Calculate the rate of reaction of the enzyme based on the graph shown in Figure 1.[2 marks]
  4. 1(b)(i)State ONE reason why curve Y is MOST likely showing enzyme activity that is inhibited.[1 mark]
  5. 1(b)(ii)Describe the action of EACH of the following inhibitors: - Organophosphate - Antabuse[4 marks]
  6. 1(b)(iii)Explain whether the type of inhibition shown in Figure 2 on page 8 is competitive or non-competitive.[3 marks]
  7. 1(c)Using Figure 3 and the provided information, explain how EACH mitochondrial protein moves molecules across the membrane differently in complete paragraphs: -…[9 marks]

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